{"id":376,"date":"2019-12-05T02:05:14","date_gmt":"2019-12-05T02:05:14","guid":{"rendered":"https:\/\/drnat.com\/nd\/?p=376"},"modified":"2019-12-05T02:05:14","modified_gmt":"2019-12-05T02:05:14","slug":"prp-and-knee-8","status":"publish","type":"post","link":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/","title":{"rendered":"PRP and KNEE"},"content":{"rendered":"<p><a title=\"Journal of orthopaedic research : official publication of the Orthopaedic Research Society.\" href=\"x-msg:\/\/129\/#\">J Orthop Res.<\/a> 2011 Sep;29(9):1320-6. doi: 10.1002\/jor.21384. Epub 2011 Mar 15<\/p>\n<h1><a href=\"x-msg:\/\/129\/pubmed\/21437966\">Autologous protein solution inhibits MMP-13 production by IL-1? and TNF?-stimulated human articular chondrocytes.<\/a><\/h1>\n<h3>Abstract<\/h3>\n<p>Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous protein solution (APS) as a potential chondroprotective agent for&nbsp;OA&nbsp;therapy. APS was prepared from platelet-rich plasma (PRP). The APS solution contained both anabolic (bFGF, TGF-?1, TGF-?2, EGF, IGF-1, PDGF-AB, PDGF-BB, and VEGF) and anti-inflammatory (IL-1ra, sTNF-RI, sTNF-RII, IL-4, IL-10, IL-13, and IFN?) cytokines but low concentrations of catabolic cytokines (IL-1?, IL-1?, TNF?, IL-6, IL-8, IL-17, and IL-18). Human articular chondrocytes were pre-incubated with the antagonists IL-1ra, sTNF-RI, or APS prior to the addition of recombinant human IL-1? or TNF?. Following exposure to inflammatory cytokines, the levels of MMP-13 in the culture medium were evaluated by ELISA. MMP-13 production stimulated in chondrocytes by IL-1? or TNF? was reduced by rhIL-1ra and sTNF-RI to near basal levels. APS was also capable of inhibiting the production of MMP-13 induced by both IL-1? and TNF?. The combination of anabolic and anti-inflammatory cytokines in the APS created from&nbsp;PRP&nbsp;may render this formulation to be a potential candidate for the treatment of inflammation in patients at early stages of&nbsp;OA.<\/p>\n<p>Copyright \u00a9 2011 Orthopaedic Research Society.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>J Orthop Res. 2011 Sep;29(9):1320-6. doi: 10.1002\/jor.21384. Epub 2011 Mar 15 Autologous protein solution inhibits MMP-13 production by IL-1? and TNF?-stimulated human articular chondrocytes. Abstract Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous protein solution (APS) as a potential chondroprotective agent for&nbsp;OA&nbsp;therapy. APS was prepared [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","ngg_post_thumbnail":0,"footnotes":""},"categories":[8,7],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>PRP and KNEE - Dr. Nathan Ehrlich, N.D.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PRP and KNEE - Dr. Nathan Ehrlich, N.D.\" \/>\n<meta property=\"og:description\" content=\"J Orthop Res. 2011 Sep;29(9):1320-6. doi: 10.1002\/jor.21384. Epub 2011 Mar 15 Autologous protein solution inhibits MMP-13 production by IL-1? and TNF?-stimulated human articular chondrocytes. Abstract Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous protein solution (APS) as a potential chondroprotective agent for&nbsp;OA&nbsp;therapy. APS was prepared [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/\" \/>\n<meta property=\"og:site_name\" content=\"Dr. Nathan Ehrlich, N.D.\" \/>\n<meta property=\"article:published_time\" content=\"2019-12-05T02:05:14+00:00\" \/>\n<meta name=\"author\" content=\"NAt\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"NAt\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/\",\"url\":\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/\",\"name\":\"PRP and KNEE - Dr. Nathan Ehrlich, N.D.\",\"isPartOf\":{\"@id\":\"https:\/\/drnat.com\/nd\/#website\"},\"datePublished\":\"2019-12-05T02:05:14+00:00\",\"dateModified\":\"2019-12-05T02:05:14+00:00\",\"author\":{\"@id\":\"https:\/\/drnat.com\/nd\/#\/schema\/person\/0fb549d14b7bb58de157ffb3998db3dc\"},\"breadcrumb\":{\"@id\":\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/drnat.com\/nd\/prp-and-knee-8\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/drnat.com\/nd\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"PRP and KNEE\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/drnat.com\/nd\/#website\",\"url\":\"https:\/\/drnat.com\/nd\/\",\"name\":\"Dr. Nathan Ehrlich, N.D.\",\"description\":\"Physician \/ Musician\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/drnat.com\/nd\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/drnat.com\/nd\/#\/schema\/person\/0fb549d14b7bb58de157ffb3998db3dc\",\"name\":\"NAt\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/drnat.com\/nd\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/68a842d3599799d23ca9a8930a405aa1?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/68a842d3599799d23ca9a8930a405aa1?s=96&d=mm&r=g\",\"caption\":\"NAt\"},\"url\":\"https:\/\/drnat.com\/nd\/author\/NAt\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"PRP and KNEE - Dr. Nathan Ehrlich, N.D.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/","og_locale":"en_US","og_type":"article","og_title":"PRP and KNEE - Dr. Nathan Ehrlich, N.D.","og_description":"J Orthop Res. 2011 Sep;29(9):1320-6. doi: 10.1002\/jor.21384. Epub 2011 Mar 15 Autologous protein solution inhibits MMP-13 production by IL-1? and TNF?-stimulated human articular chondrocytes. Abstract Catabolic inflammatory cytokines are prevalent in osteoarthritis (OA). The purpose of this study was to evaluate an autologous protein solution (APS) as a potential chondroprotective agent for&nbsp;OA&nbsp;therapy. APS was prepared [&hellip;]","og_url":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/","og_site_name":"Dr. Nathan Ehrlich, N.D.","article_published_time":"2019-12-05T02:05:14+00:00","author":"NAt","twitter_card":"summary_large_image","twitter_misc":{"Written by":"NAt","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/","url":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/","name":"PRP and KNEE - Dr. Nathan Ehrlich, N.D.","isPartOf":{"@id":"https:\/\/drnat.com\/nd\/#website"},"datePublished":"2019-12-05T02:05:14+00:00","dateModified":"2019-12-05T02:05:14+00:00","author":{"@id":"https:\/\/drnat.com\/nd\/#\/schema\/person\/0fb549d14b7bb58de157ffb3998db3dc"},"breadcrumb":{"@id":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/drnat.com\/nd\/prp-and-knee-8\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/drnat.com\/nd\/prp-and-knee-8\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/drnat.com\/nd\/"},{"@type":"ListItem","position":2,"name":"PRP and KNEE"}]},{"@type":"WebSite","@id":"https:\/\/drnat.com\/nd\/#website","url":"https:\/\/drnat.com\/nd\/","name":"Dr. Nathan Ehrlich, N.D.","description":"Physician \/ Musician","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/drnat.com\/nd\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/drnat.com\/nd\/#\/schema\/person\/0fb549d14b7bb58de157ffb3998db3dc","name":"NAt","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/drnat.com\/nd\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/68a842d3599799d23ca9a8930a405aa1?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/68a842d3599799d23ca9a8930a405aa1?s=96&d=mm&r=g","caption":"NAt"},"url":"https:\/\/drnat.com\/nd\/author\/NAt\/"}]}},"_links":{"self":[{"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/posts\/376"}],"collection":[{"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/comments?post=376"}],"version-history":[{"count":1,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/posts\/376\/revisions"}],"predecessor-version":[{"id":377,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/posts\/376\/revisions\/377"}],"wp:attachment":[{"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/media?parent=376"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/categories?post=376"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/drnat.com\/nd\/wp-json\/wp\/v2\/tags?post=376"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}